Keywords
Microbial flora, dental implants, internal chamber, infection control
How to Cite
Study of control measurement of the microbial colonization in the internal chamber of the implant and the implantabutment system by means of in vitro test. (2019). Odontoestomatología, 19(especial), 68-76. https://www.odon.edu.uy/ojs/index.php/ode/article/view/182
Abstract
Objective: To determine by means of an in vitro test, measures of control of the bioflm in the internal chamber of the implant. Method: Different antimicrobial agents were selected and they were placed in the chambers of 3 groups of implants and after 7 days immersed in a microbial suspension, we took, cultured and incubated the samples of the chambers of each study group. Results: It was found a filtration of microorganisms into the internal chamber in all the groups of implants studied, resulting in a higher count of units forming colonies (CFU) in the control group, while in the experimental groups were identified a significant reduction in the CFU count. Conclusions: A signifcant decrease in the number of CFU in the experimental groups was observed in relation to the control group, which determines the advantage of using this type of antimicrobials.
References
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18. Duarte PM, Reis AF, de Freitas PM, OtaTsukuki C. Bacterial adhesion on smooth and rough titanium surfaces after treatment with different instruments. J.Periodontol. 2009; 80(11):1824-32.
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2. Quirynen M, van Steenberghe D. Bacterial colonization of the internal part of two-stage implants. An in vivo study. Clin Oral Implant Res. 1993; 4: 158-161.
3. Broggini N, McManus LM, Herman JS, Medina RU, Oates TW, Schenk RK, Buser D, Mellonig JT, Cochran DL. Persistent Acute Inflammation at the Implant-Abutment Interface. J Dent Res. 2003; 82(3): 232-237.
4. Besimo C, Guindy J, Lewetag D, Meyer J. Prevention of bacterial leakage into and from prefabricated screw-retained crowns on implants in vitro. Int J Oral Maxillofac Implants. 1999; 14:654-660.
5. Persson L G, Lekholm U, Leonhardt A, Dahlén G, Lindhe J. Bacterial colonization on internal surfaces of Branemark system implant components. Clin Oral Impl Res.1996: 7:90-95.
6. Do Nascimento C, Barboza RE, Issa JPM, Watanabe E, Ito IY, Albuquerque Jr RF. Bacterial leakage along the implant-abutment interface of premachined or cast components. Int J Oral Maxillofac Implants. 2008; 37:177-180
7. Quirynen M, Bollen CML, Eyssen H, van Steenberghe D. Microbial penetration along the implant components of the Branemark system, an in vitro study. Clin Oral Implant Res. 1994; 5: 239-244.
8. Assenza B, Tripoldi D, Scarano A, Perrotti V, Piatelli A, Iezzi G, D´Ercole S. Bacterial Leakage in Implants with Different ImplantAbutment Connections: An In Vitro Study. J.Periodontol. 2012;83:491-497.
9. Koutouzis T, Wallet S, Calderon N, Lundgren T. Bacterial Colonization of the Implant-Abutment Interface Using an In Vitro Dynamic Loading Model. J.Periodontol. 2011;82:613-618.
10. Ricolmini A, Fernández FS, Straioto FG, Silva WJ, Del Bel Cury AA, Preload loss and bacterial penetration on different ImplantAbutment connection systems. Braz.Dent J. 2010;21(2)123-129.
11. Steinebrunner L, Wolfart S, Bobmann K, Kern M, In Vitro Evaluation of bacterial leakage along the Implant-Abutment interface of different Implant systems. Int J Oral Maxillofac Implants 2005;20:875-881.
12. Cortizo MC, Oberti TG, Cortizo MS, Cortizo AM, Fernandez Lorenzo de Mele M. Chlorhexidine delivery system from titanium/polybenzyl acrylate coating: Evaluation of cytotoxicity and early bacterial adhesion. J Dent. 2012; 40: 329-337
13. Quirynen M, De Soete M, van Steenberghe D. Infectious risks for oral implants: a review of the literature. Clin Oral Impl. Res. 2002; 13: 1-19.
14. Groenendijk E, Dominicus J, Moorer W, Van Waas M. Microbiological and clinical effects of clorhexidine enclosed in fxtures of 3i-Titamed implants. Clin Oral Impl Res. 2004; 15: 175-179
15. Santos Barboza RE, do Nascimento C, Mardegan JP, Watanabe E, Yoko I, de Albuquerque RF, Bacterial cultura and DNA checkerboard for the detection of internal contamination in dental implants. J Prosthodon. 2009; 18:376-381.
16. Koyanagi T, Sakamoto M, Takeuchi Y. Analysis of microbiota associated with peri-implantitis using 16 S rRNA gene clone library. JOral Microbiol. 2010; 24(2). Doi: 10.3402/jom.v2i0.5104.
17. Bürgers R, Gerlach T, Hahnel S, Schawartz F, Handel G. In vivo and in vitro bioflm formation on two different titanium implant surfaces. Clin Oral Implants Res. 2010; 21(2):156-64.Epub 2009,Nov,13.
18. Duarte PM, Reis AF, de Freitas PM, OtaTsukuki C. Bacterial adhesion on smooth and rough titanium surfaces after treatment with different instruments. J.Periodontol. 2009; 80(11):1824-32.
19. Lee A, Wang HL. Bioflm related to dental implants. Implant Dent. 2010; 19(5):387-93.
20. Pye AD, Lockhart DE, Dawson MP, Murray CA, Smith AJ. A review of dental implants and infection. J Hosp Infec. 2009; 72(2):104-10.Epub 2009,Mar 28.
21. Kern M, Harder S, Antimicrobial flling of implant cavities. J Prosthet Dent. 2010;103: 321-322.
22. Syed SA, Loesche WJ. Survival of human dental plaque flora in various transport media. Applied Microbiolology 1972; 24(4): 638-644
23. Gajardo M, Silva N, Gómez L, León R, Parra B, Contreras A, Gamonal J. Prevalence of Periodontopathic: Actinobacillus actinomycetemcomitans Bacteria in Aggressive Periodontitis Patients in a Chilean Population. J Periodontol. 2005; 76: 289-294.
24. Herrera D, Contreras A, Gamonal J, Oteo A, Jaramillo A, Silva N, Sanz M, Botero J, León R. Subgingival microbial profles in chronic periodontitis patients from Chile, Colombia and Spain. J Clin Periodontol. 2008;35: 106-113