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Abstract
Periodontal diseases are a major problem in human health. Decades of research have shown that the most common disease is chronic periodontitis, cha-racterized by a slow evolution with the formation of periodontal pockets, subsequent alveolar bone resorption, loss and destruction of teeth and bone tissue. While we know the multifactorial origin of the development of periodontitis, the participation of subgingival microbiota is relevant in the etiology of periodontal disease. Some pathogenic bacteria species that have been associated with the develo-pment of periodontal disease are Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Fusobac-terium nucleatum, among others. In this work we studied which of these five species were present in the periodontal pockets of 51 Uruguayan patients with chronic periodontitis. To achieve the results a conventional microbiological technique and meta-genomics (multiplex-PCR) were used. The results of the microbiological conventional technique showed the presence of A. actinomycete-mcomitans (33%) and black pigmented anaerobic bacteria (100%) in the samples. From the results obtained in the multiplex-PCR we saw that the most prevalent species were F. nucleatum (100%), T. forsythia (92%) and P. gingivalis (88%). In con-trast, lower prevalence species were P. intermedia (39%) and A. actinomycetecomitans (33%).
References
10. Ghizoni JS, Taveira LA, Garlet GP, Ghi-zoni MF, Pereira JR, Dionísio TJ, Brozos-ki DT, Santos CF, Santana AC. Increased levels ofPorphyromonas gingivalis are as-sociated with ischemic and hemorrhagic cerebrovascular disease in humans: an in vivo study. J. Appl. Oral Sci.2012;20(1): 104-112. 11. Mujica TC., Castillo-Ruiz M., Daille LK., Fuentevilla A., Bittner M. Co-detection of Periodontal Pathogens in Chilean Patients with Chronic Periodon-titis. Rev. Clin. Periodoncia Implantol.Rehabil. Oral.2010;3(3): 118-122.12. Fayad I., Lafaurie G., Contreras A., Cas-tillo D., Barón M. Microflora subgingi-val en periodontitis crónica y agresiva en Bogotá, Colombia: un acercamiento epi-demiológico. Biomédica., 2007;27(1);16-20.13. Medina A., Montoya A., Zuluaga G. Perfil microbiológico subgingival de pa-cientes con periodontitis crónica en una población de Colombia. Avances en Pe-riodoncia.2012;24(1): 47-53.14. Taba M Jr., Souza SL., Mariguela VC. Periodontal disease: a genetic perspective.Braz Oral Res.2012; 1:32-38.15. Papone V, Batlle A. Porphyromonas gin-givalis.Su relación con Periodontitis Rá-pidamente Progresiva. Odontología de Postgrado.1997;3(4): 51-55.16. Papone V, Morteo G. Un patógeno peri-odontal virulento Actinobacillus acti-nomycetemcomitans.Actas odontológicas.2005;2(1): 43-47.17. Handelsman J, Rondon MR, Brady SF, Clardy J, Goodman RM. Mo-lecular biological access to the che-mistry of unknown soil microbes: a new frontier for natural products.Chemistry&biology.1998;5(10), R245-R249.18. Rondon MR, August PR, Bettermann AD, Brady SF, Grossman TH, Liles MR, Loiacono KA, Lynch BA, MacNeil IA, Minor C, Tiong CL, Gilman M, Osbur-ne MS, Clardy J, Handelsman J, Good-man RM. Cloning the Soil Metagenome: a Strategy for Accessing the Genetic and Functional Diversity of Uncultured Mi-croorganisms. Appl.Environmental Mi-crobiology.2000;66(6): 2541-2547.19. Mullis KB, Ferré F, Gibbs R. The Polyme-rase chain reaction. Optimization of Mul-tiplex PCRs. Ed. Birkhäuser.1994; Boston.38-54. 20. Tran SD, Rudney JD. Multiplex PCR Using conserved and species-specific 16S rRNA gene primers for simultaneous de-tection of Actinobacillus actinomycetemco-mitans and Porphyromonas gingivalis.J. Clin. Microb.1996; 34 (11): 2674–2678.21. Tran SD, Rudney JD. Improved Mul-tiplex PCR using conserved and spe-cies-specific 16S rRNA gene primers for simultaneous detection ofActinobacillus actinomycetemconitans, Bacteroides for-sythus and Porphyromonas gingivalis.J. Clin. Microb. 1999;35(11):3504-3508.22. Willis SG., Smith KS., DUNN VL., Gap-ter LA., Riviere KH., Riviere GR. Iden-tification of SevenTreponemaSpecies in Health- and Disease-Associated Dental Plaque by Nested PCR. American Society Microbiology. 1999;37(3): 867-869.23. Armitage, G.Development of a classi-fication system for periodontal diseases and conditions. Annals of Peridontology .1999;4, 1-6. 24. Syed SA., Loesche WJ. Survival of Human Dental Plaque Flora in Various Transport Media. App. Microbiology.,1972; 24(4): 638-644. 25. Kolenbrander PE. Multispecies commu-nities: interspecies interactions influence growth on saliva as sole nutritional sour-ce. Int J Oral Sci.2011; 3: 49-54.26. Rosen G, Nisimov I, Helcer M, Sela M. Actinobacillus actinomycetemcomitans Serotype b Lipopolysaccharide Mediates Coaggregationwith Fusobacterium nu-cleatum.Inf Immunity.2003 71(6): 3652–3656.
27. Huang R., Li M., Gregory R. Bacterial